Journal article
Colon Cryptogenesis: Asymmetric Budding
CW Tan, Y Hirokawa, BS Gardiner, DW Smith, AW Burgess
Plos One | Published : 2013
Abstract
The process of crypt formation and the roles of Wnt and cell-cell adhesion signaling in cryptogenesis are not well described; but are important to the understanding of both normal and cancer colon crypt biology. A quantitative 3D-microscopy and image analysis technique is used to study the frequency, morphology and molecular topography associated with crypt formation. Measurements along the colon reveal the details of crypt formation and some key underlying biochemical signals regulating normal colon biology. Our measurements revealed an asymmetrical crypt budding process, contrary to the previously reported symmetrical fission of crypts. 3D immunofluorescence analyses reveals heterogeneity ..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work is supported by National Health and Medical Research Council (NHMRC) Project Grant GNT1029628, Program Grant 487922 and by funds from the Operational Infrastructure Support Program provided by the Victorian Government, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Additionally the manuscript is solely the responsibility of the institutions and individual authors and does not reflect the views of NHMRC.